Introduction: The aim of study was to assess the level of knowledge on reproductive health among the women at Puducherry and find out relationship between the knowledge on reproductive health and the selected clinical variables of women. Material and Methods: A descriptive cross-sectional research design was adapted to assess the level of knowledge on reproductive health among the women in multi-specialty Hospital, Puducherry. Consecutive sampling technique was used to select 500 women who were fulfilling the inclusion criteria. The data collection was done in regional language (Tamil) by face to face interview method through using structured interview schedule. Results: The study findings revealed that the level of knowledge on reproductive health among the 500 women, 220(44%) had inadequate knowledge, 253 (50.6%) women had moderately adequate knowledge and only 27 (5.4%) women had adequate knowledge. The mean value of knowledge regarding reproductive health among the women was 6.8 with the standard deviation of 2.4. There was statistically significant relationship found between the knowledge and history of reproductive health problems, family history of reproductive illness and use of family planning methods among the women. Conclusion: The study concluded that out of 500 women, only 27 had adequate knowledge on reproductive health. It shows that they want to improve their knowledge on reproductive health to prevent reproductive disease and promote their health. The health care providers have to take right steps promptly to reduce mortality and morbidity of the women..