Introduction: Acne vulgaris is a chronic inflammatory disorder of the skin which affects approximately 80% adolescent during puberty stage. The increasing frequency of intake of antibiotics to overcome this problem explores several side effects. Therefore it needs to focus on the herbal formulation as a topical first-line treatment. Objective: In the present study, three medicinal plants Citrus sinensis, Curcuma longa and Aloe barbadensis having significant antibacterial potential were selected to formulate a polyherbal gel for the management of acne vulgaris problem. Method: Extraction of Citrus sinensis, Curcuma longa and Aloe barbadensis was done and characterized. The topical gels were prepared which comprised extract of orange peel, aloevera, and turmeric with a different concentration. The prepared gel was kept at room temperature for 24 hours and evaluated. Result: Based on this study, polyherbal anti-acne gel showed significant antibacterial activity on Staphylococcus aureus and Staphylococcus epidermis with no irritation. The physicochemical evaluation of developed formulation showed clear, uniform and free from fibre and particle. It was also observed good spreadability and consistency with pH nearer to skin. Conclusion: Thus the study result concluded that the polyherbal gel with extract of Citrus sinensis, Aloe barbadensis and Curcuma longa with concentration 0.2%, 1% and 0.8% respectively was an appropriate formulation for the first-line topical treatment of acne vulgaris.