Introduction: Multiple variations in the origin of the vertebral artery have been reported in the literature. Objective: To study the variability in the origin and course of the vertebral arteries on both sides in both sexes and also to compare its microstructure. Methods: The vertebral artery was studied from 60 adult human cadavers on both sides. The origin and point of entry into foramen transversarium were studied and the dimensions of the artery were measured and tabulated. Results: In all 60 cadavers, the right vertebral artery (RVA) originated from the posterosuperior aspect of the first part of the subclavian artery. The left vertebral (LVA) originated from the first part of the left subclavian artery in 52 cadavers (87%). In 8 male cadavers (13%), the left vertebral artery originated directly from the arch of the aorta. The anomalous origin of LVA was longer than the right and the diameter was smaller than that of RVA. Amongst the 8 cadavers, one case appeared hypoplastic with wider close to the origin, constricted, and narrow in the middle part. In remaining cases of the normal origin of the vertebral artery, there was a significant increase in length and diameter of the vertebral artery in males compared to females on both sides. There is a predominantly increase in the length and diameter of the right vertebral artery when comparing both sexes. Conclusion: A thorough understanding of anomalous vertebral arteries is paramount when performing both diagnostic and interventional angiography.