Introduction: Ovarian cysts are a frequent finding in reproductive-aged women. Compared to laparotomy, laparoscopic cystectomy is increasingly gaining acceptance for ovarian cystectomy1 , but there is still no consensus on which approach is better regarding ovarian reserve2 . Objective: To determine and compare the impact of the approach of ovarian cystectomy whether laparoscopic or open on ovarian reserve parameters. Methodology: This prospective randomized study was conducted on 40 patients of reproductive age (range, 18__ampersandsignndash;35 years) with benign ovarian cysts who underwent either laparoscopic ovarian cystectomy (n=20) or open ovarian cystectomy(n=20). Serum anti-M__ampersandsignuuml;llerian hormone (AMH), antral follicle count (AFC), and follicular stimulating hormone (FSH)were measured preoperatively and 3 months postoperatively in all patients. Results: Serum AMH decreased significantly at 3 months postoperatively in both laparoscopic and open ovarian cystectomy groups. The AFC increased significantly at 3 months postoperatively in both laparoscopic and open ovarian cystectomy groups. On the other hand, no significant difference in FSH was found between the preoperative and 3 months postoperative levels in both groups. Conclusion: No significant difference was found between both approaches of ovarian cystectomy whether laparoscopic or open regarding the change in ovarian reserve parameters postoperatively.