Background: Acid peptic disease are a very common problem among professional truck drivers due to their lifestyle and job demands. In addition to Helicobacter pylori, Behavioural influences such as depression and diet were the primary causes of ulcers. There are other factors too. Objectives: To assess the existing knowledge regarding healthy lifestyle to prevent acid peptic disease among heavy vehicle driver and to evaluate the effectiveness of self-instructional module on knowledge regarding healthy lifestyle to prevent the same. Methods: In the present study, the quantitative research approach was used. One group pre-test post-test research design was used. Sampling technique was non-probability convenient sampling technique with 70 sample size. Result: The findings show that in pretest 14 (20 %) of study participants are having average knowledge, 33(47.14%) of study participants are having good knowledge, 23 (32.86%) of study participants are having very good knowledge whereas post-test 18(25.71%) were having very good knowledge, 52 (74.29%) had excellent knowledge. Conclusion: The study concluded that before intervention the heavy vehicle drivers have some knowledge regarding the prevention of acid peptic disease but after the intervention, they improve their knowledge. So the self-instructional module is proved to be improving their knowledge regarding prevention of acid peptic disease.