Background: Oropharyngeal cancer incidence has been increasing all over the world in the last decades despite advances in various surgical and non-surgical therapies for carcinoma treatment. The incidence of other head and neck cancers are decreasing in the world. It is now established that HPV can be contributed as a part of that increase. Objective: The present trial was aimed to investigate if the oropharyngeal cancer incidence increases with HPV, also to evaluate if the proportion of HPV-positive oropharyngeal cancer patients continues to increase, and if different treatment therapies given to patients with HPV-positive oropharyngeal cancer affect prognosis. Methods: The study was carried out 468 cases of oropharyngeal cancer,88 biopsy samples pre-treatment were available and were analysed with the PCRfrom year 2019-2020 hospital records of various cancer tertiary care centres of the Indian continent. Cases positive for PCR and p16 by immunohistochemistry were finally included in the study. Also, the detection of E7 RNA and E6 of HPV-16 was done, as these are the oncogenes that seem necessary for the oncogenesis process and also contribute to the establishment of the fact that HPV can lead to the carcinomas of the base of the tongue and oropharyngeal base. P16 immunohistochemistry was added as the diagnostic criteria to avoid misdiagnosis/overdiagnosis. Results:66 samples were tested positive for HPV 16, one sample for HPV 33, one sample for HPV 59, and one sample for HPV 35. Three samples out of these 88 were not able to be detected for HPV. mRNA expression for E6 and E7 HPV-16 was detected in all samples except 1 sample. The 88 positive patients were treated with radiotherapy (40), chemoradiotherapy (20), or accelerated radiotherapy (28). No difference was noticed concerning the overall survival rates with the three different treatment modalities used. Conclusion: The results showed that the percentage of HPV positive patients with oropharyngeal cancer increased exponentially from one year to another in consecutive years. No difference was noticed about the overall survival rates with the three different treatment modalities used including radiotherapy, chemoradiotherapy, or accelerated radiotherapy.