Introduction: Sleep also is considered a time when other body system restores their energy and repairs their tissues and is important to wellbeing and optimal health. Lack of sleep is reason for many mental conditions in all stages of human lives specially during studentship. Objective: To evaluate the factors associated with quality of sleep among undergraduate physiotherapy students. Methods: Undergraduate physiotherapy students aged 18-25 years and studying in Nootan College of Physiotherapy, Visnagar and SPB Physiotherapy College, Surat were explained about the procedure and informed consent was obtained. Participants were asked to fill the hard copy of Pittsburgh Sleep Quality Index (PSQI) for assessment of the sleep quality, Depression Anxiety Stress Scale (DASS) for assessment of the depression, stress and anxiety. Participants were asked to fill the questionnaire. Body Mass Index (BMI), total minutes of physical activity done by participant per week and the total duration of use of electronic devices during bedtime of each participant were recorded. Results: Regression analysis shows that obesity contributes to 0%, depression contributes to 9%, stress contributes to 13.7%, anxiety contributes to 10.6%, physical activity contributes to 2.4% and use of the electronic device during bedtime contributes to about 2.2% in affecting sleep quality. Conclusion: Our results support the idea that sleep quality in physiotherapy students is notably associated with several psychological factors like Stress and Anxiety. Hence, Psychological Stress and Anxiety should be evaluated and treated for good quality of sleep in undergraduate physiotherapy students. There is a need to do further study with a large sample size to check various factors associated with poor sleep quality.