Background: Impaired wound healing is a disastrous medical problem associated with chronic diseases and ageing. To accelerate skin regeneration many techniques have been researched laser and LED has been used for these purposes. Topical phenytoin is simple to use, safe, inexpensive and readily available drug and plays a significant role in the rate of healing of wounds. Objective: We aimed to evaluate irradiation of a LED light 670-nm for wound healing. Using ulcer mice model with and without the combination of phenytoin drug. Methods: Four groups 24 mice per group received treatment as follows. Group I: Ulcers followed up without any treatment modality.Group II: Ulcers subjected to topical Phenytoin spray twice daily, Group III: Ulcers irradiated with red LED 670 nm at a fluence of 5 J/cm2 . Group IV: Ulcers treated by combined topical Phenytoin spray and red LED670 nm at a fluence of 5 J/cm2 . All treatments started from the 1st day postoperatively and for up to three consecutive weeks or till complete healing of the ulcer. Results: The results showed the fastest healing in the combined group with more deposition of collagen fibres, larger amounts of granulation tissue, less oedema. The second best treatment was the red LED 670-nm only treated group as mice showed less evident features with fewer collagen fibres deposition. Conclusion: Red LED 670nm combined with phenytoin is an effective enhancer of wound healing that stimulates the secretion of growth factors in the wound bed and induce many changes during the skin healing process, especially in favouring neo collagen fibres to be better organized and led more deposition of collagen fibres.