Introduction: Organ donation is life-saving for patients with end-organ failure but unfortunately organs are not available for transplantation due to poor knowledge, attitude, myths and misconceptions towards organ donation. Shortage of organ donation can be solved by increasing awareness regarding organ donation. The present study was conducted in M.G.M. Medical College, Aurangabad, Maharashtra to assess the knowledge and attitude of first-year medical students towards organ donation. Objectives: To study the knowledge __ampersandsignamp; attitude of first-year medical students towards organ donation. To identify the areas where knowledge was lacking __ampersandsignamp; augmenting the knowledge of that area. Methods: A cross-sectional study was conducted at M.G.M. Medical College, Aurangabad, Maharashtra by distributing the self__ampersandsignndash;structured questionnaire to first-year medical students. The data was collected and analyzed statistically after the approval of the Ethics Committee for Research on Human Subjects (MGM-ECRHS/2020). Results: 130 students had participated in this study after taking informed verbal consent. 98.46% of students knew about organ donation. 53.84% claimed the source of information about organ donation as television and internet while 70.76% claimed as discussion in medical college and 20% as radio. The awareness regarding eye, kidney, skin, heart and liver was 86.92%, 86%, 72.30%, 72.30% and 72.30% respectively.92.30% of students were aware of laws regarding organ donation. Conclusion: Medical students had a high level of awareness and a positive attitude towards organ donation. However to have in-depth knowledge __ampersandsignlsquo;organ donation and transplantation__ampersandsignrsquo; must be included in the medical curriculum.