Background: Urinary tract infection is one of the most common bacterial infections encountered by paediatricians. Currently, the diagnosis and management of acute urinary tract infection and recurrent urinary tract infection in children remains controversial. Prompt diagnosis and initiation of treatment are important in preventing long-term renal scarring. However, increasing antibiotic resistance may delay the initiation of appropriate therapy. Antibiotic prophylaxis remains controversial. Objective: To identify the bacterial pathogens involved in pediatric UTI and study their antibiogram patterns. Methods: A total of 1492 urine samples of pediatric patients (0-14 years), clinically suspected of UTI, were processed in the Department of Microbiology for 3 years. Urine samples were collected in a sterile container and processed by inoculating on cysteine lactose electrolyte deficient agar (CLED). Antibiogram was performed by disc diffusion method as per CLSI guidelines. Gram-negative isolates were studies for expended spectrum __ampersandsignbeta;-lactamase (ESBL) production and S. aureus isolates were screened for methicillin-resistant S. aureus (MRSA). Results: Out of 1492 samples, 876 (58.7%) were found to be culture positive. Gram-negative bacteria (80.4%) comprised the maximum number of isolates. E. coli (60.7%) was the most frequently isolated uropathogen, followed by K. pneumoniae (13.2%) and S. aureus (11.2%). Twenty-one per cent of E. coli and 17.4% of K. pneumoniae were ESBL producers. Among S. aureus, 32.6% were MRSA. Conclusion: High-level antimicrobial resistance was observed in pediatric UTI. We should adopt antimicrobial use based on local epidemiological data which helps in maximizing clinical outcome.