Background: Raised intraocular pressure (IOP) and reduced ocular perfusion pressure (OPP) are significant risk factors for glaucoma. It is important to study the factors which affect IOP and OPP, to prevent the development of glaucoma. Some of the factors include age, gender, systemic blood pressure, obesity etc. Though few studies have demonstrated the gender difference in IOP in normotensive individuals as well as in glaucoma patients, the results are inconsistent. It has also been proved that hypertension is more prevalent in males. Hence the present study was taken up to explore the gender difference in IOP levels among individuals with prehypertension, a predictor of hypertension in future. Objective: To compare and evaluate the IOP and OPP values among males and females with prehypertension. Methods: 100 voluntary participants with prehypertension (systolic blood pressure of 120__ampersandsignndash;139 mmHg or diastolic blood pressure of 80__ampersandsignndash;89 mmHg) were recruited from the ophthalmology clinic in Mysuru in the age group of 20 - 50 years. They were categorised into two groups based on their gender. Written informed consent was taken. IOP and blood pressure were recorded using rebound tonometer and sphygmomanometer respectively. Results: Males had a significantly higher IOP in both the eyes when compared to their female counterparts. There was no difference in their OPP values. Conclusion: Since elevated IOP is seen in males than females, IOP could be a leading indicator for early diagnosis of glaucoma in males.