Background: Oral carcinoma is the most common and sixth cause of death worldwide. AURKA gene encodes Aurora kinase-A, cell-cycle regulated protein which is involved in microtubule formations and stabilization of spindle poles during chromosome segregation. Overexpression of the gene is often associated with chromosome instability. Objective: The study investigates the association of AURKA gene polymorphism in oral squamous cell carcinoma (OSCC) progression. Methods: The present study follows an observational study design which employs in-silico tools to assess the frequency of the alleles related to the polymorphism (rs2064863) in different populations and its possible consequences. Results: The polymorphism (rs2064863) of AUKRA gene selected was an intron variant with the minor allele frequency of 0.37 for the G allele. Interestingly, the ancestral population or the African population demonstrated a frequency of 83% for the T allele and 16% for the G allele, where other populations such as American, European and South Asian populations presented with different allele frequencies in which both the alleles occur with similar frequencies in the population. Conclusion: The present study revealed the possible reasons for the positive selection of the minor allele in these populations, which could provide clues on the possible association of this variant with OSCC in the south Asian population.