Introduction: Glyoxalase-1, an erythrocyte enzyme of the glyoxalase system catabolizes the methylglyoxal is the main precursor of advanced glycation end products (AGEs). Elevated methylglyoxal and AGEs are strongly associated with the development of diabetic complications. The association between trace elements and glyoxalase-1 in diabetic patients is not well established. Objective: To assess the concentration of serum Mg, Se, and Mn in diabetic patients and compare these in different groups defined in terms of duration of the DM. Methods: The present study compares the concentration of micronutrients (magnesium, selenium, and manganese) in non-complicated (group A __ampersandsignle;4 years) and complicated (group B __ampersandsigngt;4years) diabetes mellitus type-2 patients. Sixty-four (50.8%) patients were in group A, and 62 (49.2%) patients in group B. This cross-sectional study also correlates the concentrations with glyoxalase-1 levels. Results: Glyoxalase-1 has shown higher concentration in group B than A (50.65__ampersandsignplusmn;5.32 vs. 41.29__ampersandsignplusmn;3.58 ng/mL; p-value __ampersandsignlt;0.001) where all the micronutrients have shown higher levels in group A. One-way ANOVA test showed a significant association between glyoxalase-1 and glycosylated Haemoglobin, magnesium, and selenium (p__ampersandsignlt;0.05). Conclusion: Glyoxalase-1 is a predictor of poor glycemic control in diabetic patients, and it is associated with the early development of a diabetic complication. The concentrations of serum magnesium, selenium, and manganese decrease with the increased duration of diabetes.