Introduction: Betel nut consumption with or without tobacco and betel nut infested with Aspergillus flavours, generates free radicals and reactive oxygen species, which in turn induces a high rate of lipid peroxidation. Serum Malondialdehyde (MDA) is a highly recognized marker of lipid peroxidation. Objective: The present study was conducted to evaluate the effect of turmeric on increased lipid peroxidation by using serum MDA level __ampersandsignamp; as a surrogate outcome on clinical signs __ampersandsignamp; symptoms of oral submucous fibrosis (OSMF). Material and Methods: Total of 150 subjects was divided into 4 groups. Group A: 30 subjects suffering from OSMF were treated with turmeric and counselled for stoppage of habit, Group B: 30 subjects suffering from OSMF were treated with turmeric and NOT counselled for the cessation of habit, Group C: 30 subjects suffering from OSMF did not keep on turmeric medication but counselled for stoppage of habit, Group D: 30 cases were betel-nut habitual without OSMF and Group E: 30 healthy control subjects. Results and Observations: Statistically significant reduction of Serum MDA levels post-treatment in group A patients as compared to group B __ampersandsignamp; group C (p__ampersandsignlt;0.05). Mean difference was observed as 9.00 in group A, 3.81 in group B, 2.62 in group C. Conclusion: Increased serum Malondialdehyde (MDA) in OSMF would serve as a valuable surrogate marker in early diagnosis, treatment, and prognosis. The antioxidant properties of turmeric reduce the oxidative stress even with the effects of areca alkaloids and tobacco. Serum MDA level was decreased significantly in subjects taking turmeric medication for 4 weeks.