Aim: In the present scenario of environmental consciousness the requirement of environment friendly textiles is increased. Plant extracts and oils demonstrating antifungal properties may provide protection for textile application. Methodology: This study was to evaluate the antifungal properties of natural plant extracts, such as essential oils, for se on wood. Three essential oils were evaluated for their ability to inhibit growth of Aspergillus Niger, Trichoderma Reesis. Lemon grass oil, pepper mint oil and pine oil inhibited growth of fungi. Cotton fabric is chosen for the study and the selected oils are applied on to the fabric by dip dry method. Result: The treated fabric is tested with two fungal pathogens(Aspergillus niger, Trichoderma reesis). It shows the better antifungal properties. Also the fabric show better result for physical test. Conclusion: These findings support the application of essential oils for surface treatment or vapour exposure of textile to prevent fungal infection and fungal growth.