Introduction: Infertility is a major reproductive health problem today in which Male infertility may be contributory to 30 to 40 % of infertile couples. Musalyadi Churna is most commonly utilized classical Ayurvedic formulation in the management of impotency and infertility which are a major increasingly worldwide concern. It is a combination of herbal drugs like Musali (Chloro-phytum borivilianum), Vidarikanda (Pueraria tuberosa DC), Guduchi Tinospora cordifolia (Willd Miers ex Hook. f. and Thoms.), Kapikacchu (Mucunna prurita Hook.), Gokshura (Tribulus Terrestris Linn.), Shalmali (Bambax malabaricum DC) and Amalaki(Phyllanthus Emblica Linn.). Methods: Musalyadi Churna will be prepared as per the reference of classical text Sharangdhar Samhita for pharmaceutical study and then its analytical study will be done. In experimental studies To assess the toxicity of Musalaydi churna, its acute oral toxicity on Wistar/SD rats will be performed using OECD 423 guidelines; Genotoxicity and Antigenotoxicity will be performed using Chromosomal aberration assay on 36 Swiss albino mice and Spermatogenesis study will be conducted on 60 Wistar/SD male rats as per OECD guidelines and scientific research articles. Expected Results: Observations of pharmaceutical and experimental studies will be noted and presented in the form of tables, chart, photographs etc. Conclusion: As the ingredients of Musalyadi churna are easily available and the method of preparation does not require much time and manpower, therefore if this formulation showed significant efficacy then this will open a new gateway for the management of infertility.