Objective: This study aims to determine differences in levels of Tumor Necrosis Factor-__ampersandsignalpha; (TNF-__ampersandsignalpha;) in pregnant women with preeclampsia in the Bugis, Makassar, Mandar, and Toraja tribes so that they can be used as a basis for initial consideration to detect tribal variations as a risk factor for preeclampsia (PE). Method: This study is an observational a case-control design conducted in March-July 2020 in several health centers and hospitals at Lasinrang. The number of samples used in this study was 88 people, 44 pregnant women with preeclampsia, and 44 normotensive pregnant women. The criteria of the sample in this study were pregnant women __ampersandsigngt;20 weeks with a diagnosis of preeclampsia with blood pressure __ampersandsignge;140 / 90mmHg and were not experiencing other pregnancy complications. Data collected includes age, parity, and Body Mass Index (BMI). TNF-__ampersandsignalpha; levels were assessed using the Human TNF-__ampersandsignalpha; ELISA Kit, Bioassay Technology Laboratory. Results: There were significant differences in TNF-__ampersandsignalpha; levels in preeclampsia pregnant women where the highest TNF-__ampersandsignalpha; levels of pregnant women preeclampsia were in Makassar (181.12 __ampersandsignplusmn; 36.27 Pg/ml) while the Mandar tribe with the lowest TNF-__ampersandsignalpha; levels (Makassar 143.30 __ampersandsignplusmn; 22.74 Pg/ml). While differences in TNF-__ampersandsignalpha; levels in preeclampsia and normotensive pregnant women in each tribe found significant results in the Makassar tribe (p 0.05). Conclusion: There are differences in TNF-__ampersandsignalpha; levels in preeclampsia pregnant women and normotensive in the Makassar and Toraja tribes. So that tribal variation can be considered as a risk factor for preeclampsia pregnant women.