Background: Hospitalization of the neonate is very stressful and challenging for mothers as they have to adapt to their own physiological and psychological changes along with the unexpected needs of the baby. If these needs are not identified and taken care off can lead to suboptimal health and developmental outcomes for the infant. Purpose: To assess the maternal needs and priorities of the hospitalized neonate in NICU. Methods: A cross-sectional survey was conducted among 61 mothers of hospitalized neonates with the use of maternal needs inventory. Needs were categorized into five deduced dimensions Assurance, proximity, Information, support and comfort. Besides, Mothers were asked to enlist five most important needs as per the priority from high to low. Results: Out of 25 listed needs, Assurance needs were reported as most important and comfort needs as a least. In priority, all five needs were related to the health of the baby than the mother__ampersandsignrsquo;s comfort. Significant association between maternal needs and selected demographic variables were observed in the study. Conclusion: Maternal need inventory is a simple yet effective tool for identifying the needs of mothers which is vital for promoting family-centred care based on dignity and respect; information sharing; participation; and collaboration.