Objective: This study evaluates the image acquisition efficacy of current available dental imaging modalities for human bite marks in forensic odontology. Dental casts were collected retrospectively from Riyadh dental Hospital. Methods: Human bite marks were registered on different materials like pink wax, aluwax and Polyvinyl siloxane. These study casts with their corresponding bite marks were imaged and categorized into: (1) 2D imaging using digital cameras following the American Board of Forensic Odontology guidelines; (2) 2D CBCT imaging with Cone-beam computed tomography (CBCT); and (3) 3D imaging using CAD/CAM and CBCT. Sensitivity, specificity and receiver operating characteristics (ROC) curves were traced for observers in all the approach. Paired t-test and kapa tests were utilized for the inter-observer consistency. Each modality had a significant difference under the ROC plotted. Results: The study observed that 3D CBCT imaging in comparison with the other imaging modalities had a marginally higher value of P= 0.0002. The kappa method for the inter-observer agreement was used to confirm this observation. Conclusion: This study clearly shows the advantage of 3D imaging using CBCT in human bite mark imaging. Based on the results, a 3D forensic odontological image database collection with a gold standard is the future potential of this study.