Background: Drug-Resistant Tuberculosis (TB) resurrection over the globe broadens the research towards its causative agent Mycobacterium tuberculosis. The diarylquinoline derivative drug Bedaquiline which inhibits ubiquitous enzyme ATP synthase was introduced in 2012. ATP synthase is an essential enzyme that helps in supplying energy to Mycobacterium tuberculosis even in the unfavourable conditions which helps them to survive in macrophages of human beings. Since 2017, resistance to bedaquiline has been reported worldwide which emphases us for the discovery of the new drugs. The medicinal plant Tinospora cordifolia is used in various traditional healing systems worldwide to treat diabetes, leprosy, etc. Methods: The current study summarizes the pharmacology of the phytocompounds of Tinospora cordifolia. In silico study was performed by using tools like CASTp, PubChem, ChemSpider, Swiss ADME, Pro Tox server, HEX dock, PyMol, and Lig plot+. ADMET study of the phytocompounds was also studied. Results: The results obtained in the study indicated that the 2-Hydroxy-3-oxoicosanoate was the potent inhibitor which shows the highest affection towards ATP synthase of Mycobacterium tuberculosis and also accomplishes the entire criterion to be an effective drug. Conclusions: Many of the phytocompounds of Tinospora cordifolia shows good ADMET analysis and docking result. Among all the phytocompounds 2-Hydroxy-3-oxoicosanoate shows the best result in terms of docking. This is only an in-silico study to fasten the drug discovery process, further validation of the proposed drug requires in-vitro and in-vivo study.