Introduction: The wide-ranging positive effects of phytoecdisteroids suggest use them as drugs for the treatment of diabetes and related diseases. Among phytoecdysteroids, a well-expressed hypoglycemic effect is known for turke sterone. Objective: We aimed to determine the effect of turke sterone on the endocrine and exocrine parameters of the pancreas in alloxan-induced diabetic rats. Material and Methods: White outbred rats were divided into 4 groups to receive saline or a single dose of alloxan (170 mg/kg, intraperitoneal) and/or turkesterone (10 mg/kg/24 h) and glibenclamide (5 mg/kg/24 h) for 10 days. For histological analysis the tail portion of the pancreas was kept in formalin for 3 days, dried in ethanol, parafinized and then stained with hematoxylin-eosin. The glucose, insulin, C-peptide and total protein level in serum, as well as __ampersandsignalpha;-amylase activity in serum and pancreas homogenate, were identified by using special kits (Human. Germany). Results: Diabetes caused complete destruction of the islet apparatus and partial destruction of the pancreatic acini tissue. Treatment with turkesterone of alloxan-induced diabetic rats resulted in restoration of the histostructure of endo- and exocrine pancreas, increase of serum glucose as well as decrease insulin, C-peptide, and total protein level. In addition, turkesterone treatment of alloxan-induced diabetic rats led to an approximation of __ampersandsignalpha;-amylase activity of pancreas and serum to control value. Conclusion: This study has shown that turkesterone led to improving the exocrine and endocrine function in alloxan-indused diabetic rats.Hence, it can be recommended for further testing as an anti-pancreatic and anti-diabetic drug in humans.