Diabetes mellitus has emerged rapidly due to the societal factors which influence the large population by their lifestyle. Initially, it was known as __ampersandsignlsquo;a disease of a rich man__ampersandsignrsquo; but in recent years it affects all class of people, As we know the medical therapy in started through ancient and traditional medicine from plant origin and there is extensive ancient history if embedded in the area of plant medicine. Gliricidia sepium belongs to the family Fabeacae. Firstly this particular plant was found in Central America after that many of the tropical countries employed these plants as aremedy for different disorders as a folore medicine. __ampersandsignalpha;-Amylase Inhibition Assay: It is the common method to estimate the amount of reducing sugars in different types of test samples. In this essay, we can measure the amount of reducing sugar generated after-treatment of the test solution with the __ampersandsignalpha;-amylase enzyme. __ampersandsignalpha;-glucosidase Inhibition Assay: This method involves the reaction of the sample with glucosidase enzyme and p-nitrophenyl glucopyranoside in this assay as concentration is high sample solution or standard solution the lower the absorbance. The findings of the current project indicated that selected plant exerted moderate inhibition in both methods. Maybe they isolate fractions may give clear and productive results