Introduction: Allergic rhinitis (AR) is a Type 1 IgE mediated hypersensitivity disorder, characterized by sneezing, rhinorrhea, nasal congestion, itching, and postnasal drip. Recently, many studies reported that vitamin D plays a critical role in Allergic rhinitis however, this association yet remains unclear. Aim __ampersandsignamp; Methodology: The study aims to evaluate total nasal symptom scores (TNSS), serum IgE, serum absolute eosinophil count (AEC) in patients of AR, pre and post-treatment with and without supplementation of vitamin D. The randomized, open-labelled, parallel-group study, including 100 patients randomized in 1:1 allocation. Group A received fluticasone nasal spray alone, group B received vitamin D (1000 IU once daily) plus fluticasone nasal spray for 3 weeks. The results of TNSS, serum IgE, serum AEC in patients with AR, pre and post-treatment with and without supplementation of vitamin D were compared and evaluated. Results: The mean difference pre and post-treatment in TNSS (group A= 6.553, group B= 7.551), serum IgE (A= 81.05, B= 109.92), serum AEC (A= 79.29, B= 97) showed no statistical significant difference between two groups (P-value __ampersandsignge; 0.05). Conclusion: In this study, supplementation of vitamin D did not alter the natural course of Allergic rhinitis. Though changes in the symptoms and lab values were noted in between the groups, it was statistically not significant which would prove beneficial for the patients. __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp; __ampersandsignnbsp;