Aim: Tuberculosis remains the most deadly infectious killer affecting about one-quarter of the world population. Early diagnosis and correct detection of TB has always been a challenge since its discovery in 1882. The economic burden in low- and middleincome countries is estimated to be US$ 10.1 billion in 2019. It is necessary to analyze the current tuberculosis diagnostic techniques and understands their advantages and limitations for future advancement. Observation: The current status of tuberculosis necessitates advancement in diagnostic techniques. In recent years with the initiatives taken by WHO, various new diagnosis techniques have been introduced to the world. Techniques such as Fluorescent microscopy and bleach microscopy with their improved sensitivity are proposed to give a new face to the microscopy. Whereas molecular techniques such as Cartridge based NAAT and line probe assay are also proving their potential. Culture techniques such as MB/BacT, Versa TREK/ESP, and Thin layer agar cannot be replaced for their sensitivity and specificity, but they are required to be used alongside new techniques. Novel techniques such as biosensors with the aid of their higher sensitivity, low detection cost, and simple methodology are paving a better future for the world. Conclusion: Despite the long list of conventional diagnostic techniques, early and precise diagnosis of tuberculosis is still a challenge in developing countries. For the complete eradication of tuberculosis from world, we need to focus on developing new diagnostic techniques and also increase awareness about the existing techniques.