Context: Coagulase negative Staphylococci (CoNS) are Gram positive cocci that are widespread commensals among mammalia. CoNS are more resistant to antimicrobials, including __ampersandsignszlig;-lactam antibiotics, some hospitals revealing oxacillin resistance rates approaching 90%. Aim: Determine the prevalence and antimicrobial susceptibility profile of CoNS in our hospital, and to observe various risk factors responsible for the isolation of clinically significant species. Setting and Design: This prospective study was done in the Department of Microbiology, SKIMS, JandK over a period of 1year Material and Methods: A total of 325 CoNS isolates were obtained from patients of all age groups and both the sexes. Antimicrobial susceptibility testing was done by Kirby-Bauer disc diffusion method. The minimum inhibitory concentration of vancomycin and teicoplanin for CSCoNS, was done by microbroth dilution method. Statistical Analysis: The Chi-square test was used to compare two groups. Results: Out of 325 CoNS recovered, 140 (43.1%) were found to be clinically significant. Maximum CSCoNS were isolated from the age group 0-9 years 27 (19.3%) and blood samples (n=48, 34.3%). Samples from the neonatal intensive care unit yielded the maximum number of CSCoNS, 29 (20.7%). Hospital stay of __ampersandsigngt;1 week, prior use of __ampersandsignbeta;-lactam antibiotics and fluoroquinolones and intravenous line catheters were significant risk factors in patients from whom CSCoNS were recovered. Staphylococcus epidermidis was most common isolate. Methicillin resistance was seen in 79 (56.4%) of CSCoNS. Conclusion: The recovery of CoNS should be seriously regarded as they are resistant to multiple antibiotics and their prevalence not only limits the treatment options but also acts as a reservoir of drug-resistant genes.