Introduction: Following menopause, decreased oestrogen production from ovaries leads to lipoprotein profile derangement, drastic alterations in the metabolism of glucose and insulin, distribution of body fat, coagulation and fibrinolysis and vascular endothelium dysfunction. Oestrogens include many cardioprotective mechanisms that alter the vascular tone by enhancing the levels of nitrous oxide. Endothelial cells are stabilized by oestrogens, they augment antioxidant potential and alter fibrinolytic proteins. These are all cardioprotective mechanisms that are reduced with the beginning of menopause. Objectives: To evaluate the relation of oestradiol hormonal variation with plasma lipid concentrations in pre- and postmenopausal women. Material and mMethods: 50 premenopausal women and 50 postmenopausal female were selected as subjects. Data were obtained via clinical assessment from laboratory investigations and questionnaires. Results: Serum levels of Total Cholesterol (TC), Triglycerides (TG), LDL-cholesterol, and VLDL-cholesterol in postmenopausal women were significantly elevated when matched with premenopausal women. For postmenopausal women, the level of HDLcholesterol declined substantially. The concentration of estradiol in post-menopause women was significantly lower (p__ampersandsignlt;0.001). Conclusion: Menopause results in alterations in the lipid profile by reducing HDL and increasing total cholesterol (TC), triglycerides (TG), LDL-cholesterol and VLDL-cholesterol, thus raising the chances of cardiovascular disease. These shifts are due to decreased amounts of oestrogen that are observed in menopause.