Aim: This study aimed to evaluate the cognizance, attitude, practice, prevalence of Needlestick, and sharps injuries among dental professionals at Nellore, India. Materials and Methods: Cross-sectional study of 276 dental professionals were given a questionnaire and were informed about the purpose of the study and the study design by using a Convenience sampling technique. Third-year and final year UG students, dental house surgeons, postgraduate students, and dental practitioners were included in the survey as dental professionals. Pretested and predesigned questionnaire forms gave to dental professionals. Statistical analysis was performed by employing the statistical package, SPSS version 13. A comparison of these variables between the dental health care professionals made using Pearson__ampersandsign#39;s Chi-square test. Results: 96% of dental professions considered Needle Stick Injury (NSI) Needle Stick Injury transmit diseases and 59% of dental professionals knew about the Universal Precaution Guidelines (P __ampersandsignlt; 0.05). In case of an accidental NSI, 64.4% of the dental professionals reported that they would have first contacted a medical emergency room, 85.96 % did not report because the injury was due to sterile needle. 80.70% had an occurrence of NSI during device use. 72% followed one-handed needle recapping technique or scoop technique, and 36.95% used needle burner and syringe destroyer to dispose of a needle. Conclusion: Dental practitioners and postgraduates were with adequate knowledge regarding NSIs. Third-year, Final year undergraduate students and Dental house surgeons had poor knowledge regarding NSIs.