Introduction: Human rotavirus group A genotyping is important to monitor changes in rotavirus genotypes distribution. Although multiplex reverse transcription polymerase chain reaction (RT-PCR) assays are widely used for genotyping of rotavirus strains, they may be less sensitive than monoplex RT-PCR assays Objective: To use monoplex nested and semi-nested RT-PCR for re-examination of human rotavirus group A common G and P genotypes respectively in two hundred and fifty stool specimens and twenty-four raw sewage samples which were examined in a previous study for human rotavirus group A common G and P genotypes using multiplex nested and semi-nested RT-PCR respectively. Results: Increasing the number of typeable G and P specimens were observed in this study in comparison to the previous study. Rotavirus common G genotypes were detected in 57.60% (144/250) of the positive clinical specimens. The most frequent G genotype was G1 (29.60%), followed by G3 (23.20%). Both of them were with a significantly higher prevalence than there prevalence in the previous study. Genotypes G2 and G4 which were absent in the previous study had appeared in this study. Genotype G2 had been detected in 2.80% of the specimens, while genotype G4 had been detected in 2.00% of the specimens. In the raw sewage samples, genotype G1 was detected in a higher percentage (37.50%) than the previous study. Genotypes G2 and G4 were detected in 4.17% for each of them, while they were absent in the previous study. Rotavirus common P genotypes were detected in 92.80% (232/250) of the positive clinical specimen. The most frequent P genotype was P[4] (41.60%) followed by P[8] (24.00%), P[6] (20.40%). All of them were with significantly higher prevalence than their prevalence in the previous study. In the raw sewage samples, genotype P[4] was detected in higher percentage (41.67%) than the previous study. Conclusion: This study has shown that nested and semi-nested monoplex RT-PCR is more sensitive than nested and seminested multiplex RT-PCR in genotyping of human rotaviruses.