Introduction: Burn wound injuries are one of the most common, invasive and devastating forms of trauma. Despite the recent advances in burn wound management, bacterial infections persist as an important complication and leading cause of morbidity and mortality among burnt patients. Identification and antimicrobial susceptibility pattern of bacterial pathogens associated with burn wounds can help clinicians to plan patient management effectively and efficiently. Materials __ampersandsignamp; Methods: This prospective study was conducted for a period of one year (July 2016- June 2017). A total of 160 specimens (wound swabs and pus exudates) from burn wound patients received at microbiology department for culture and sensitivity were included in the study. Once received the samples were processed immediately as per the standard operating procedures of our laboratory. Identification and antimicrobial sensitivity testing of the bacterial isolates was performed on VITEK__ampersandsignreg;2 COMPACTautomated identification and antimicrobial susceptibility testing (ID/AST) system (bioMerieux, France) and interpreted as per Clinical Laboratory Standards Institute guidelines. The use of automated VITEK__ampersandsignreg;2 COMPACT system for ID/AST ensures accurate results for most of the clinical isolates and eliminates the requirement of human analysis and error of results. Results: A total of 160 samples were received from burn wound patients, out of which 113 (70.6%) were culture positive. P.aeruginosa, A.baumannii and Proteus mirabilis were the most predominant gram-negative isolates whereas S.aureus, Coagulase-negative staphylococcus and Str.pyogenes were the most commonly isolated gram-positive organisms. Antimicrobial sensitivity profile of bacterial isolates revealedPiperacillin-tazobactam, imipenem, cefoperazone-sulbactam and colistin to be the most effective antimicrobials against gram-negative isolates, whereas linezolid, teicoplanin, vancomycin and amikacin were the most effective drugs against gram-positive isolates. Conclusion: Due to the increased morbidity and mortality associated with the burn wound infections, early detection of the causative agents and the intervention are a prerequisite for better clinical outcomes of burnt patients. Data extrapolated from our study can be helpful for primary care physicians to optimize the treatment modalities, articulating policies for empiric antimicrobial therapy and to minimize the rate of infection among burn wound patients.