Background: Study is designed to see correlation of cardiac specific Troponin I and Creatine Kinase-MB level to diagnose ST segment elevation myocardial infarction. Aim and Objectives: To access the diagnostic importance of cardiac specific Troponin I over Creatine Kinase-MB in patient of ST-segment elevation myocardial infarction. Materials and Methods: A comparative study was carried out in 100 patients, having ST segment elevation myocardial infarction. A group of 100 normal healthy individuals, age and sex matched from the same population served as controls. Troponin I and Creatine Kinase-MB levels were estimated in both the groups. Results: Results of present study shows that cardiac specific Troponin I and CK-MB levels were significantly higher in STEMI patients compared to healthy subjects with __ampersandsignlsquo;p__ampersandsignrsquo; value of __ampersandsignlt;0.0001. Pearson__ampersandsignrsquo;s correlation test show positive correlation between Troponin I and CK-MB in STEMI patients. 17% of STEMI patients had normal CK-MB levels, but there Troponin I levels were elevated significantly. Conclusion: Troponin I level is more specific for confirming the diagnosis of acute myocardial infarction where CK-MB fails to do so.