Context: Burden of diabetes mellitus is increasing gradually along with its complications. Nowadays along with fasting and post-prandial plasma glucose (FPG and PPPG), HbA1c is included in the diagnosis and assessing prognosis. The current practice is samples for HbA1c and FPG, PPPG are collected in different vials (EDTA and Fluoride vial respectively). Aims: Present study was designed to find out whether any difference occurs or not in HbA1c values when collected in EDTA __ampersandsignamp; fluoride vials __ampersandsignamp; also to find out the stability of HbA1c in fluoride vials for five consecutive days. Settings __ampersandsignamp; Design: This case control study was conducted at service laboratory of Dept. of Biochemistry at NRS Medical College __ampersandsignamp; Hospital Methods __ampersandsignamp; Material: Samples were collected from 107 subjects (44 male __ampersandsignamp; 63 female) in both EDTA (E) __ampersandsignamp; fluoride vials (F). Estimation of HbA1c was done from both the vials separately by Latex Turbidimetric and chromatography method (HPLC). 10 randomly chosen samples were collected and stored at 4oC in fluoride vials. HbA1c estimation was done from F vial for 3 consecutive days. Statistical Analysis: The results were tabulated and analyzed using software. Results: The HbA1c values from both EDTA __ampersandsignamp; Fluoride vials were well correlated and no statistically significant differences (p= 0.69) existed among E __ampersandsignamp; F vials. ANOVA analysis showed no significant differences (p-0.99) among HbA1c level in Fluoride vial for 3 consecutive days. Conclusions: Estimation of blood glucose level __ampersandsignamp; HbA1c level can be done from the fluoride (F) vial without compromising the quality of reports.