Background: Nature has been a source of medicinal agents for thousands of years and a striking number of modern drugs have been isolated from natural sources, many based on their use in traditional medicines or phytomedicines. As the plants have co-evolved with pathogens, they have also developed the chemical protection pathways against the organisms. Therefore, varieties of plants compounds are associated with antimicrobial and antibacterial activities. The hibiscus rosa sinensis plant extract have multiple organic component like flavonoids, tannins, alkaloids, triterpenoids which are known to have antibacterial activity against E. coli , P. aeruginosa, Salmonella species. Phytotherapy is considered to be less toxic and minimal or no side effects in comparison to modern allopathic medicines. Therefore, in today__ampersandsignrsquo;s scenario there has been reappearance of interest developed in herbal medicine. Therefore, the study was planned to look for antibacterial activity of extract of petals and leaves of Hibiscus rosa sinensis against Material and Methods: Escherichia coli isolated from different clinical samples along with resistant antibiotics. All resistant isolates were tested along with Hibiscus Rosa Sinensis extract both leaves and petals extract with resistant antibiotics. Results: We found that among 35 (68.63%) resistant E. coli isolates to different antibiotics and 85.72% were from urine samples. Enhancement effect in zone size with many resistant antibiotics was seen individually with both extracts. Both extracts showed equal enhancement in zone size of cefotaxime (20.83%) and amikacin (25%). No effect of extracts was seen on levofloxacin.