Aim: To extract Tyrosine ammonia Lyase(TAL) from Trigonella foenum-graecum and utilize it for the treatment of depigmentation by carrying out in-vivo analyses on Zebra fish embryos Tyrosine ammonia Lyase is an enzyme that is involved in deamination of L-tyrosine to P-coumaric acid. The enzyme TAL was extracted from Trigonella foenum-graecum. This enzyme has been targeted to be used in depigmentation of the skin in the current study using zebra fish as a model organism. This enzyme participates in depigmentation by inhibiting the production of melanin pigment, where in conversion of L-tyrosine in the melamine synthesizing pathway has been prevented by deaminating L-tyrosine to P-coumaric acid. The methodology of separation of proteins using acetone has been implemented in the extraction of the enzyme. Standardization of enzyme was accomplished to detect the presence of the enzyme. The formation of P-coumaric acid indicated the presence of the enzyme that was detected utilizing UV-Visible spectrophotometer at 380nm. In-vivo experimental study was carried on the zebra fish embryos for detecting the presence of the enzyme that was confirmed by observing the depigmented patterns in the developed embryos that were been treated with TAL.