Background: Diabetes is one of the common metabolic disorder that occurs due to poor secretion of insulin. It is more common among aged people in India. The incidence of diabetes is increasing every day and this indicates the increasing need for the treatment for diabetes. Objective: The objective of the present study is to screen the effects of selected substituted dihydroxyflavone for its in vitro antidiabetic effect by finding the potential to inhibit the enzymes __ampersandsignalpha;-Glucosidase. Materials and Methods: The dihydroxy flavones used in the present study includes 2__ampersandsignrsquo;,3__ampersandsignrsquo;- dihydroxy flavone and 2__ampersandsignrsquo;, 4__ampersandsignrsquo; dihydroxy flavones. They were synthesized using standard procedures. In vitro __ampersandsignalpha;-glucosidase inhibitory activity was evaluated by Li et al., 2004. The different concentration of the flavonoid ( 0.1, 0.3, 1, 3,10, 30, 100, 300, 1000 (__ampersandsignmu;M/ml) were used and the experiment was done for triplicate sample. The standard antidiabetic drug used in the study was Acarbose. The inhibitory activity was calculated and tabulated. Results: The selected Dihydroxy flavones 2__ampersandsignrsquo;,3__ampersandsignrsquo;- dihydroxy flavone and 2__ampersandsignrsquo;, 4__ampersandsignrsquo; dihydroxy flavones showed significant invitro anti diabetic activity when compared with standard drug acarbose. The IC50 value of 2__ampersandsignrsquo;,3__ampersandsignrsquo;- dihydroxy flavones and 2__ampersandsignrsquo;, 4__ampersandsignrsquo; dihydroxy flavones was found to be 0.47__ampersandsignmu;M/ml, 46.37 __ampersandsignmu;M/ml respectively.