Objectives: 1. Assess impact of Computer Assisted Learning (CAL) as animal simulator in practical Pharmacology on 2nd MBBS students 2. Evaluate feedback responses of students __ampersandsignamp; faculty to CAL Methods: Study was conducted in Pharmacology department of a tertiary care hospital. Students of 2nd MBBS and faculty members of Pharmacology department of this institute participated of the in the study. CAL sessions on __ampersandsignldquo;effect of drugs on rabbit eye__ampersandsignrdquo; were conducted using software of Animal simulator of Reed Elsevier India. Students took pre and post MCQ tests. Feedback was obtained from students and faculty through questionnaire and interviews. Statistical analysis was done by student__ampersandsignrsquo;s __ampersandsignlsquo;t__ampersandsignrsquo; test (p value __ampersandsignlt; 0.5) Observations and Results: Significant improvement in performance of students was seen in post- test. Average score increased from 48% to 77% after CAL. Range of score also improved. Most of students and faculty gave positive feedback about CAL in feedback questionnaire and interviews. Most of students opined that CAL helped to improve their understanding and was enjoyable due to AV impact. Faculty felt that most of the students were motivated in session and CAL would help them to retain knowledge about drug. Conclusion: CAL is interesting, informative and motivating. It helps students to understand concepts about practical Pharmacology. Faculty opined that CAL is a good tool and should be included in regular teaching learning of practical Pharmacology.