The current study was conducted to assess the major compositional parameters like total phenolic , anthocyanin and total flavonoid content and also to assess the effect of strawberry polyphenol extract on the antioxidant potential using DPPH and ORAC assay, carbohydrate hydrolyzing enzymes i.e __ampersandsignalpha;-amylase, __ampersandsignalpha;-glucosidase inhibition activity and ACE inhibition activity spectrophotometrically. The total phenolic content in the strawberry polyphenol extract was observed to be 10 mg GAE per ml of extract. Total flavonoid content was estimated approximately 526__ampersandsignplusmn;0.88 __ampersandsignmu;g quercetin per ml of extract and total anthocyanin content was observed to be 164__ampersandsignplusmn;1.15 __ampersandsignmu;g cyaniding-3- glucoside equivalent per ml of extract. These major compositional components have a contribution towards the biofunctional properties like antioxidant, ACE inhibitory and antidiabetic properties. The antioxidant activity measured by DPPH and ORAC assays was observed to be 2.76__ampersandsignplusmn;0.01 mM, 8.3__ampersandsignplusmn;0.15 mM, respectively at the polyphenol concentrations 0.5 mg/ml. The percentage inhibition activity of carbohydrate hydrolyzing enzyme i.e __ampersandsignalpha;-amylase, __ampersandsignalpha;- glucosidase and ACE (angiotensin converting enzyme inhibitory activity) of polyphenol extract was observed to be 6.33__ampersandsignplusmn;1.45, 25.33__ampersandsignplusmn;0.88 and 24.16__ampersandsignplusmn;0.44 at 0.5 mg/ml polyphenol concentration. Results predicted the health promoting attributes of strawberry polyphenol extract. Hence, these can be supplemented into the diet of human beings, as the synthetic drugs leads to bad effects on the human health.