Objectives: The aim of the present study is to evaluate hepatoprotective effect of Picrorhizakurroaextracts (PKE) against Fumonisin b1 (Fb1) induced hepatotoxicity. Methods: Hepatoprotective analysis was done by estimating serum biochemical parameters Alanine aminotransferase (ALT) and aspartate aminotransferase (AST), antioxidant enzyme status lipid peroxidations, ROS generation, and protein carbonyls were also estimated to know the oxidative damage. Further protein expressions were confirmed by western blotting. Results: The mice treated with 100 and 200 mg/kg b.wt PKE, showed a significant reduction in serum AST and ALT to 175.41 __ampersandsignplusmn; 7.82, 68.52__ampersandsignplusmn;1.57 and 126.64 __ampersandsignplusmn; 1.72, 57.41__ampersandsignplusmn;1.28 units/ml of serum respectively. The decrease in antioxidant levels with Fb1 was significantly restored with 100 and 200 mg/kg by wt PKE. The lipid peroxidation products with Fb1 treatment was also reduced with PKE supplementation. The Fb1 induced ROS was reduced with 60 and 140% by 100 and 200 mg/kg/ by wt PKE administration. The PKE restored CAT and GPx enzyme/protein levels and further down regulated caspase 8 and caspase 3 expression. Conclusion: The present observations suggested that the treatment with PKE extract enhance the recovery from Fb1 induced hepatic damage due to its antioxidant and hepatoprotective property.