Background: The role of trace elements in hemodialysis (HD) patients has not yet been clearly recognized. To minimize the health consequences of persistent HD, the levels of trace elements should be seriously adjusted. Methods: The study was across-sectional conducted in Khartoum state ofSudan between August 2013 and April 2015 included 150patients with end stage renal disease treated with hemodialysis and 75 healthy volunteers as control group. Informed consent was assigned by each participant before taking the blood sample. All samples were analyzed for magnesium, zinc and copper. Results: Compared with healthy controls, in hemodialysis patients average serum levels of zinc (Zn) (663 __ampersandsignmu;g/L) and copper (Cu) (797__ampersandsignmu;g/L) were insignificantly different; however, magnesium (Mg) levels (38.61 mg/L) were significantly increased. According to__ampersandsignnbsp;sex of ESRD patients, serum Mg level was increased significantly in females, while insignificant changes were observed in Culevel. This study stated that Zn, Cu and Mg levels were significantly increased in elder patients more than younger ones. Zn, Cu and Mg were increased as increasing duration of dialysis, changing significantly in serum Zn and Cu. Conclusions: Our results concluded that in hemodialysis, aging, and sex have significant effects on Zn, Cu and Mg.