Aim: To compare various diagnostic methods for diagnosing Extra Pulmonary Tuberculosis. Methodology: A total of 173 extra pulmonary specimens depending on the site of infection were collected aseptically in sterile containers for tuberculosis (TB) culture and__ampersandsignnbsp;Polymerase Chain Reaction (PCR)__ampersandsignsbquo;__ampersandsignnbsp;and in formalin for the purpose of cytology and__ampersandsignnbsp;pathology. TB culture was performed using the MGIT 960 liquid system__ampersandsignsbquo;__ampersandsignnbsp;after initial screening using Light Emission Diode (LED) microscopy and ZN staining__ampersandsignsbquo;__ampersandsignnbsp;while all the samples were also processed by Gene Xpert . Results: Gene Xpert showed a high sensitivity of 85.71%__ampersandsignnbsp;and negative predictive value (NPV)__ampersandsignnbsp;of 98.67%__ampersandsignnbsp;while LED-FM had a high specificity of 98%__ampersandsignsbquo;__ampersandsignnbsp;which was the same as of ZN stain and a high positive predictive value (PPV)__ampersandsignnbsp;of 86.36%. Discussion: Clinical presentation of Extrapulmonary tuberculosis (EPTB)__ampersandsignnbsp;is atypical;__ampersandsignnbsp;especially when the disease involves obscure occult sites and EPTB may not even be considered in the initial list of differential diagnosis. Diagnosis of extrapulmonary tuberculosis is challenging due to the paucibacillary nature as well as atypical clinical presentations. Definitive diagnosis of tuberculosis involves demonstration of M. tuberculi by microbiological__ampersandsignsbquo;__ampersandsignnbsp;cytopathological or histopathological methods. Conclusion: Extrapulmonary tuberculosis diagnosis should hence be made using more than one diagnostic methods. In our present study, Light Emission Diode-__ampersandsignnbsp;Fluorescent Microscopy (LED-FM)__ampersandsignnbsp;proves to be a sensitive__ampersandsignsbquo;__ampersandsignnbsp;specific and cost effective method for diagnosis of extrapulmonary tuberculosis..