Introduction: Diabetes mellitus and its complications have become a major health problem for the public of South East Asians especially in India. Exposure to organophosphorus pesticides which are very much in use for the agriculture purpose worldwide produces disorders of glucose homeostasis, because there is increasing evidence of environmental pollutants especially pesticides to the development of insulin resistance and type2 diabetes mellitus. Background: To study the significance of Secreted Frizzled Related Protein4 (SFRP4) levels in both diabetic and non diabetic rural agriculture population who are exposed to pesticides for the prediction of diabetes mellitus. Total 46 Type 2 Diabetic patients and 42 non diabetic subjects were included in this study. Results: The Pearson correlation coefficient was used to find the correlation between SFRP4 and Glycated hemoglobin (HbA1c) and Fasting glucose. And the correlation was found between SFRP4 levels with HbA1c (R=0.65) and also found between SFRP4 and fasting glucose levels (R=0.54) and both are statistically significant (p-__ampersandsignlt;0.05). Conclusion: If properly intervene the population with proper health education regarding precautionary measures for the use of pesticides and awareness of diabetes who have a strong family history of diabetes mellitus with upper normal level after testing this new marker SFRP4, we can delay or even may reduce the incidence of diabetes mellitus in the rural population due to the exposure of pesticides.