Aim: The aim of present study is to understand the mechanism of calcification and deposition of how protein and carbohydrate deposits in Diabetic and Non Diabetic induced Nuclear Cataract Lenses. Methods: This Experimental comparative study conducted on Twenty six cataract samples after Small Incision Cataract Surgery (SICS) collected and stored in formalin and saline solution to avoid contamination. The Cataract lens specimens were dried for one week at 25oC, under specific Relative Humidity (RH) conditions, and then used for spectral investigation to determine its biophysical characteristics and molecular composition by using FTIR spectroscopy and X-ray Diffraction technique. Results: FTRI technique on the diabetic nuclear cataracts we have found unsaturated aldehydes groups are found at a frequency of 1688(narrow peak) and also aldehydes are found at a frequency of 2823 (medium peak) and XRD technique resulted that there is difference in the presence of electron density in different samples and mainly in the diabetic nuclear cataracts and non diabetic nuclear cataract. Conclusion: We can understand that if we can be able to reduce aldehydes groups__ampersandsignrsquo; formation in the diabetic cataract lenses then we can be able to reduce the formation of cataract in diabetic patients. The X-ray Diffraction techniques conclude that Diabetic Lenses have different electron density which is different from Non Diabetic Cataract lenses. Clinical Significance: The significance of this study explained about molecular bonding and aldehyde groups in the diabetic cataract lenses. Hence we found that this technique and further experiments may shed light and give us the better understanding on the Calcification in diabetic cataractous crystalline lens.