The vegetable oils (non-edible) have proved to be promising feedstock for the biodiesel production. The present study deals with the evaluation of Simarouba , Jatropha and Pongamia for their growth performance from the seed germination to the first bearing, production and characterization of oil and biodiesel as they are considered as biofuel crops capable to grow on waste lands. Variations in bioproductivity of the plants were compared every six months for four years of investigation and biodiesel parameters were also studied and analyzed statistically. Identification of better genotype or superior phenotype having better yield and oil content is the important step in the tree improvement strategies involved in the biofuel program. The results obtained from the studies on the bioproductivity traits, their correlation and satisfying biodiesel quality has proved a Simarouba species as a potential candidate to provide sustainable feed stock for biodiesel industries.