Introduction: Congenital hydrocolpos and the hydrometrocolpos is a rare disorder caused by accumulation of cervico-vaginal secretions that present with cystic pelvic mass in neonates.Prenatal diagnosis is important to prevent complications like sepsis and renal failure and to affect prognosis. We will report a case of a newborn female admitted to our NICU with abdominal mass and imperforated anus diagnosed to have hydrometrocolpos, septated vagina with bicornate uterus and urogenital sinustreated surgically. Case Report: This report will help us to develop a high index of suspicion of hydrometocolpos in female newborn with abdominal mass.