Aim: Main objective of the study is to characterize and evaluate invitro cytotoxicity of T-2 toxin isolated from corn. Methodology: The fungal strain was isolated by standard blotter method; morphological identification was done using scanning electron microscope (SEM). The fungal strain was identified as F. sporotrichioides based on its cultural and morphological characteristics and internal transcribed spacer (ITS/ITS4) primers. The toxin was characterized using thin layer chromatography (TLC), HPLC and Fourier Transform Infrared Spectroscopy (FTIR) Result: 100ml of culture filtrate yielded 10.126__ampersandsignmu;g of toxin. The functional groups of toxin were identified by (FTIR) method. Invitro cytotoxicity of T-2 toxin was evaluated on human keratinocytes, hepatoma and neuron cell lines. HaCaT and HepG2 cells were found to be more sensitive than SHSY5Y to T-2 toxin exposure at 50-100ng and 100-200ng. Discussion and conclusion: T-2 toxin was extracted and characterized from corn samples, by analytical and molecular methods in addition FTIR analysis verifies the functional groups of T-2 toxin. Further invitro cytotoxicity was evaluated using different human cell lines. Comparably skin and liver cell lines found more sensitive to T-2 toxin exposure. Sensitivity of the cell lines to the toxin at ng may be due to the purity of the toxinKey Words: Plasma Fibrinogen, Haemodialysis, Diabetes.