Background: Iron overload being one of the major adverse effect related to blood transfusion in __ampersandsignbeta; Thalassemia major,leads to various endocrinal disorders among which hypogonadism is noteworthy.Pituitary or gonadal iron deposition or both may lead to this. Objectives: The main purpose of this study was to evaluate the effect of iron overload measured by serum ferritin on pituitary gonadotrophins (FSH, LH) and gonadal steroids (Estrogen in females,Testosterone in males) levels and the correlation between them in pubertal thalassemic subjects(cases). Methods: Serum harvested from blood samples collected from 30 cases(15 males,15 females) and 30 controls were used to estimate Serum ferritin, LH, FSH, Estradiol in females and Testosterone in males. Results: The serum LH (p__ampersandsignlt;0.001), FSH (p__ampersandsignlt;0.001), Estradiol in females (p 0.002) and Testosterone in males (p 0.002) were significantly low in cases than controls. There was significant negative correlation between Ferritin:FSH (__ampersandsignrho;-0.706, P__ampersandsignlt;0.001- females, __ampersandsignrho;-0.838, P__ampersandsignlt;0.001-males), Ferritin: LH (__ampersandsignrho;-0.885, P__ampersandsignlt;0.001-females, __ampersandsignrho;-0.806, P__ampersandsignlt;0.001-males) Ferritin: Estradiol (__ampersandsignrho;- 0.584, P__ampersandsignlt;0.001-females), Ferritin: Testosterone ( __ampersandsignrho;-0.664, P__ampersandsignlt;0.001-males) and significant positive correlation between FSH:LH (__ampersandsignrho;0.623, P__ampersandsignlt;0.001-females, __ampersandsignrho;-0.871, P__ampersandsignlt;0.001-males), FSH: Estradiol (__ampersandsignrho;0.839, P-0.01- females), FSH: Testosterone (__ampersandsignrho;-0.860, P-0.001-males), LH: Estradiol (__ampersandsignrho;0.913, P__ampersandsignlt;0.001-females, LH: Testosterone (__ampersandsignrho;-0.849, P__ampersandsignlt;0.001-males). Conclusions: The current study reveals that iron overload has significantly reduced pituitary gonadotrophins as well as gonadal sex steroids,but the effect being more due to iron deposition in pituitary as indicated by the higher rho(__ampersandsignrho;) value between ferritin and LH, FSH than between ferritin and estradiol, testosterone. The deficiencies of gonadotrophins have failed to stimulate the gonads leading to hypogonadotrophic hypogonadism and delayed puberty.