Aim: The main objective of this investigation was to show the variation of Andrographolide content in Andrographis paniculata from thirty seven different sites of Balaghat (Jabalpur MP) region. Methodology: In order to study the variation in andrographolide content, plant material was collected from different sites and were analyzed through quick and accurate HPLC chromatographic method using C-18 column (4.6 x 250 mm, 5__ampersandsignmu; particle size). Result and Discussion: The average andrographolide content varied from 0.01 to 1.2%. Highest percent of andrographolide in Andrographis paniculata were estimated from site Kera of south Balaghat 1.2%. Lowest content of andrographolide in Andrographis paniculata were estimated from the site of Salhen north Balaghat region that is 0.02%. Conclusion: The content of this active ingredient in this plant varies with in the plant parts and with the geographical distribution.