Background: Chronic liver disease leading to cirrhosis, hepatocellular carcinoma or hepatic failure have been associated with Hepatitis B and C virus infections. Detection of various viral markers of Hepatitis B have been reported to be useful tools to assess the progression of disease. In view of chronic liver disease being high in this population, an attempt was made to detect Hepatitis B viral markers and antibodies to Hepatitis C in patients reporting to this hospital. Methods: Serum samples from a cross section of hundred clinically confirmed cases of chronic liver disease were screened for HBsAg, total anti-HBc, total anti-HBs and HCV Ab. The patients who were positive for HBsAg were further screened for HBeAg, total anti HBe and IgM anti-HBc. Genotyping of Hepatitis B virus was done by nested PCR. Results: HBsAg positive patients accounted for 10% of the patients admitted to this hospital. HCV antibodies were also seen in 10% of the patients. A single patient tested positive for both HBsAg and HCV antibodies. Anti HBs was present in 11%, total anti HBc was detected in 42% and 87.5% had anti HBe. Two patients had IgM to HBc. Genotype C was detected in all nine HBV DNA positive samples. Conclusion: Hepatitis B and C markers may be used to detect recent infection, active replication and carriers among cases of chronic liver disease. Genotype C is associated with faster progression to hepatocellular carcinoma, hence there is a need to monitor these patients.