Use of barrier membranes for the regeneration of bone defects has significantly changed implant dentistry in the past years. The design of the membranes employed in this study plays an important role in establishing a healing environment by separating tissues during healing and thus providing space for regeneration of bone in cases of insufficient bone around implants, it acts like a tent in cases of extraction sites- which is a potential site for future implant placement thus reducing the problems of irregular ridge defect, and the rigidity of the material prevents tissue ward collapse of the membrane. The present study was conducted to prepare the titanium in the form of foil, and tested for biocompatibility using fibroblast cells, hydroxyapatite coating was made on the foil, characterization by X-ray diffraction for both the uncoated and the coated foils was done, and osteoblasts adhesion test was done and viewed under scanning electron microscope both before and after the test. Materials and methods: Commercially pure titanium foils were made using 16% hydrofluoric acid. 3__ampersandsigntimes;3 cm sheets were cut and dipped in the acid intermittently and alternatively dipped in water and wash the acid. For every minute of etching the material is measured using a micrometer to check the thickness of the foil. It is carried on until it is 60__ampersandsignmu; thick and uniform pores appear. Hydroxyapatite coating was done over the foil by dip coating in a solution made by a mixture of hydroxyapatite powder, water, glycerine, polyethylene glycol, and ethanol. The foil is dipped and withdrawn at 45? a speed of 10mm/ 15 seconds. Then the foil is dried in an oven at 100 ? and heat treated in a microwave oven for 45 minutes. Results: The titanium foil was treated for biocompatibility using fibroblast cells, and the test showed that the material was biocompatible. After coating with hydroxyapatite, the uncoated and hydroxyapatite-coated titanium foils and the hydroxyapatite powder were characterized by x-ray diffraction and the dip coated foils clearly showed the hydroxyapatite peaks along with the substrate titanium peaks. Scanning electron micrographs of the uncoated foil revealed uniform distribution of through and through pores and scaly appearance in between pores. The coated foil revealed uniform distribution of hydroxyapatite coating. And the thickness of the hydroxyapatite in cross section was 50__ampersandsignmu;. Osteoblasts adhesion test was conducted on both the uncoated and the coated foils and scanning electron microscopic study was conducted. The micrographs revealed the adhered osteoblasts on the surface of both the uncoated and the coated test samples. Conclusion: A new membrane material titanium foil was prepared by acid etching, and the material proved biocompatible in fibroblast culture study. The foil was dip coated with hydroxyapatite. X-ray diffraction showed foils with hydroxyapatite peaks along with the substrate titanium peaks. Scanning electron microscopic study revealed micro pores and scaly appearance of the uncoated foil, and for the coated foil it revealed a uniform coating of the hydroxyapatite. The surface roughness of the foil has given provision for the attachment of the INDIGENOUS UNCOATED AND HYDROXYAPATITE COATED COMMERCIALLY PURE TITANIUM FOILS FOR GUIDED BONE REGENERATION IN DEFECT SITES OF IMPLANTS __ampersandsignndash; AN IN VITRO STUDY Ranzani. R., Abby Abraham, Chakravarthy.R., Lakshmi.S., Department of Prosthodontics, Meenakshi Ammal Dental College,Chennai __ampersandsignndash;600 095, India E-mail of Corresponding Author: drchakra19@gmail.com 110 International Journal of Current Research and Review www.ijcrr.com Vol. 04 issue 17 September 2012 hydroxyapatite. Osteoblasts adhesion test was done and scanning electron microscopic study was conducted to view the adhered cells. The osteoblasts cells have adhered to the substrate of both the uncoated and the coated test samples. The results revealed that this study could give rise to a new generation of osseo conductive membranes for use in implant defect sites.