Aim: Assessment of genome integrity in testis and to explain the reasons for infertility with the drug metosartan. Methodology: Comet assay with freshly isolated mitochondria, cryopreserved mitochondria and cryopreserved tissue mitochondria and cell proliferation assay by dye exclusion method and topoisomerase assay in fresh and deep freezed DNA. Results and Discussion: Comet assay of isolated mitochondria both from fresh and cryopreserved samples was examined by fluorescence microscope, which revealed that cryopreservation of testes mitochondria samples lead to injury to the tissue and DNA fragmentation. Topo isomerase assay has showed that increased incubation leads to DNA fragmentation and cell proliferation assay with the drug has showed the 0% viability of the cells by trypan blue. So, in vivo studies are necessary to confirm whether the drug is safe or not. Conclusion: Assessment of genome integrity lead to the finding that drug was not safe it resulted in so many difficulties in invitro condition.