This study was conducted to ascertain the attitudes of undergraduate pharmacy students to animal experimentation after they had completed experiments on frog abdominal rectus muscle, goat intestine, studied the effect of various drug like analgesic, anti-inflammatory, diuretics etc on mice and rats and on computer programme in academic year 2008-09. Barely sixty five percent of the students agreed with to use alternative of life animals like mice and rats for purpose in education, an overwhelming majority (seventy four percent) felt that the animal experiments involved needless pain and suffering to the animals. A large majority of students felt the need to reduce the number of animals by demonstrating the practical, to explore alternatives to the animal experiments, and to restrict animal experiments for research on life threatening diseases. More than half of the students were unaware of the government regulations on animal experiments. Students considered, understanding physiological processes as the main objective of animal experiments. The results of this study indicate that there is a need to modify the curriculum taking into account the development and availability of the new technology. The students also need to be made aware of the Government regulations on animal experiments particularly the role of the CPCSEA and the institutional animal ethics committee.